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Induction of cytolytic cells by DB was sensitive to sonication and freeze-thawing as determined by CD3epsilon -redirected lysis analysis. In accordance with that, induction of virus neutralizing antibodies was much more effective when untreated DB were used as immunogen.

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In , however, came the unexpected and terrible news that his son had died in Rome during his own Italian journey. Goethe fell seriously ill immediately but recovered.

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